Characterisation And Application Of Synthesised Chitosan Nanoparticles Against Pseudomonas.aeruginosa Bacteria

Abstract

ABSTRACT Chitin is a potential energy source as well as gene and drug delivery carrier, and in the emerging field of nanobiotechnology. Chitosan, the product of chitin deacetylation, is known for its various uses in different fields. Due its increasing advantages, the chitosan nanoparticles has still importance in research purposes. Chitosan is a natural, biocompatible, biodegradable, nontoxic and easily available polymer that can be used to prepare nanoparticles. Chitosan nanoparticles can be widely used in pharmaceutical industries as an antimicrobial agent. The present study focuses on the synthesis of chitosan nanoparticles its Characterisation and application. Chitosan nanoparticles are synthesised into desired size and surface charge using sodium Tripolyphosphate (STPP) initiated ionic gelation process. These chitosan nanoparticles were characterised by UV- VIS spectroscopy to know the kinetic behaviour that ranges between 200 to 700 nm. The antimicrobial properties of chitosan nanoparticles were studied as its application and it was done in Pseudomonas.aeruginosa bacteria. The organism were identified by Gram's staining and biochemical test . Their activity was tested using well diffusion method and zone of inhibition was noted. The Zone of inhibition obtained against Pseudomonas aeruginosa were 2.3cm for 10mg/ml, 2.5cm for 20mg/ml and 2.8cm for 30mg/ml.The study is thus a good demonstration of the applicability of chitosan nanoparticles as an effective antimicrobial agent.