Abstract:
ABSTRACT
Chitin is a potential energy source as well as gene and drug delivery carrier, and in the
emerging field of nanobiotechnology. Chitosan, the product of chitin deacetylation, is known
for its various uses in different fields. Due its increasing advantages, the chitosan nanoparticles
has still importance in research purposes.
Chitosan is a natural, biocompatible, biodegradable, nontoxic and easily available polymer
that can be used to prepare nanoparticles. Chitosan nanoparticles can be widely used in
pharmaceutical industries as an antimicrobial agent. The present study focuses on the synthesis
of chitosan nanoparticles its Characterisation and application. Chitosan nanoparticles are
synthesised into desired size and surface charge using sodium Tripolyphosphate (STPP)
initiated ionic gelation process. These chitosan nanoparticles were characterised by UV- VIS
spectroscopy to know the kinetic behaviour that ranges between 200 to 700 nm. The
antimicrobial properties of chitosan nanoparticles were studied as its application and it was
done in Pseudomonas.aeruginosa bacteria. The organism were identified by Gram's staining
and biochemical test . Their activity was tested using well diffusion method and zone of
inhibition was noted. The Zone of inhibition obtained against Pseudomonas aeruginosa were
2.3cm for 10mg/ml, 2.5cm for 20mg/ml and 2.8cm for 30mg/ml.The study is thus a good
demonstration of the applicability of chitosan nanoparticles as an effective antimicrobial agent.
